Women in Country Music will unite for a historic opening performance at next week's 53rd Annual CMA Awards. The number, led by Carrie Underwood, Reba McEntire and Dolly Parton, will also feature Terri Clark, Sara Evans, Crystal Gayle, The Highwomen (comprised of Brandi Carlile, Natalie Hemby, Maren Morris and Amanda Shires), Martina McBride, Jennifer Nettles, Tanya Tucker and Gretchen Wilson, each performing iconic Country songs for a decades-spanning medley never before seen.

The CMA Awards will also feature an additional performance from Kelsea Ballerini, joined by fellow breakthrough artists Lindsay Ell, Maddie & Tae, Ashley McBryde, Carly Pearce and Runaway June. They will be performing a CMA Song of the Year and CMA Single of the Year featuring a special appearance by Little Big Town.

The 53rd Annual CMA Awards, hosted by Carrie Underwood with special guest hosts Reba McEntire and Dolly Parton, celebrates legendary women in Country Music throughout the broadcast. The show airs live from Bridgestone Arena in Nashville on Wednesday, Nov. 13th at 8 p.m. ET on ABC.